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New England Biolabs components
Components, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+components/Q5+Reaction+Buffer/pm41723172-288-14-20
Average 99 stars, based on 1826 article reviews

components - by Bioz Stars, 2026-11

99/100 stars

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Recombinant:

Article Title: Continuously tunable multistability in DNA replication networks.
Article Snippet: .. Magnesium chloride hexahydrate (MgCl2·6H2O) and tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) were purchased from Aladdin Chemical Reagent Co., Ltd. Hydrochloric acid (HCl) was sourced from Sinopharm Chemical Reagent Co., Ltd.. Nt.BbvCI nicking endonuclease (10,000 U·mL−1, 2.3 μM), RNase H (5,000 U·mL−1), 10× rCutSmart® buffer (1× buffer components: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg·mL−1 recombinant protein, pH 7.9@25°C), Klenow Fragment (3→5 exo-) (5,000 U·mL−1), deoxyribonucleotide triphosphates (dNTPs), and NEBuffer 2 (1× buffer components: 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM dithiothreitol, pH 7.9@25°C) were purchased from New England BioLabs Inc.. .. Ammonium persulfate substitute (APS substitute), Trisborate-EDTA (TBE, 5× premixed powder), Gel-Red (10000×), acrylamide/bis-acrylamide (29:1, 30% solution), tetramethylethylenediamine (TEMED) substitute and DNA loading buffer were purchased from Beyotime Biotechnology.

Article Title: Continuously tunable multistability in DNA replication networks
Article Snippet: .. Magnesium chloride hexahydrate (MgCl 2 ·6H 2 O) and tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) were purchased from Aladdin Chemical Reagent Co., Ltd. Hydrochloric acid (HCl) was sourced from Sinopharm Chemical Reagent Co., Ltd. Nt.BbvCI nicking endonuclease (10,000 U mL −1 , 2.3 μM), RNase H (5000 U mL −1 ), 10× rCutSmart® buffer (1× buffer components: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg mL −1 recombinant protein, pH 7.9@25 °C), Klenow Fragment (3→5 exo-) (5000 U mL −1 ), deoxyribonucleotide triphosphates (dNTPs), and NEBuffer 2 (1× buffer components: 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl 2 , 1 mM dithiothreitol, pH 7.9@25 °C) were purchased from New England BioLabs Inc. .. Ammonium persulfate substitute (APS substitute), Tris-borate-EDTA (TBE, 5× premixed powder), Gel-Red (10,000×), acrylamide/bis-acrylamide (29:1, 30% solution), tetramethylethylenediamine (TEMED) substitute and DNA loading buffer were purchased from Beyotime Biotechnology.

Purification:

Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.
Article Snippet: .. Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH2O The PCR conditions were as follows: (1) initial heating at 98 ̊C for 30 seconds, (2) 14 cycles of 10 seconds at 98 ̊C and 60 seconds at 72 ̊C, (3) A final extension at 72 C̊ for 60 seconds. .. AR TI CL E IN P RE SS The six reactions were pooled into a single sample and purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013), eluted into 20 μl of ddH2O, and quantified using a Qubit fluorometer (Thermo Scientific).

Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
Article Snippet: .. Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH 2 O. .. The PCR conditions were as follows: (1) initial heating at 98 °C for 30 s, (2) 14 cycles of 10 s at 98 °C and 60 s at 72 °C, (3) A final extension at 72 °C for 60 s. The six reactions were pooled into a single sample and purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013), eluted into 20 μl of ddH 2 O, and quantified using a Qubit fluorometer (Thermo Scientific).

Polymerase Chain Reaction:

Article Title: CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning
Article Snippet: .. After the supplementation of the remaining reaction components (BbsI, NEB buffer, Dithiothreitol, T7 Ligase, ATP) as specified for the 7-fragment assembly (Table ) or the 9-fragment assembly (Table ), the reactions were incubated in temperature cycling at 37 °C 5 min / 16 °C 5 min for 50–80 cycles using a PCR cycler (BIO-RAD T100 Thermal Cycler). .. To ensure the breakdown of remaining non-assembled plasmid backbone, and additional reagents (i.e. Exonuclease V (RecBCD), NEBuffer, ATP) were added in the same PCR tube followed by 30 min incubation at 37 °C.

Article Title: CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.
Article Snippet: .. After the supplementation of the remaining reaction components 671 (BbsI, NEB buffer, Dithiothreitol, T7 Ligase, ATP) as specified for the 7-fragment 672 assembly (Table 2) or the 9-fragment assembly (Table 3), the reactions were 673 incubated in temperature cycling at 37 °C 5 min / 16 °C 5min for 50-80 cycles 674 using a PCR cycler (BIO-RAD T100 Thermal Cycler). .. To ensure the breakdown of 675 remaining non-assembled plasmid backbone, and additional reagents (i.e. 676 Exonuclease V (RecBCD), NEBuffer, ATP) were added in the same PCR tube AR TIC LE IN PR ES S 677 followed by 30 min incubation at 37 °C.

Amplification:

Article Title: Development of visual dye-based loop-mediated isothermal amplification assays for Mycobacterium avium subsp. paratuberculosis detection in ruminants.
Article Snippet: Additionally, the MgSO4 (Thermo Fisher Scientific, USA) concentration was tested at 4, 6, 8, and 10 mM, whereas the dNTP (Thermo Fisher Scientific) concentration was varied at 0.6, 1.0, 1.4, and 1.8 mM. .. The other reaction mixture components were fixed as 10× Isothermal Amplification Buffer II (New England Biolabs [NEB], USA); 8 units of Bst 2.0 WarmStart DNA Polymerase (NEB); 1.6 μM each of FIP (Flc+F2) and BIP (Blc+B2), 0.2 μM of F3 and B3, and 0.4 μM of LF and LB primers; 0.8 mM betaine (Thermo Fisher Scientific); and 150 μM HNB along with 1 μL template DNA (25 μL total volume). ..

Incubation:

Article Title: Multi-layered dosage compensation of the avian Z chromosome by increased transcriptional burst frequency and elevated translational rates
Article Snippet: .. Afterwards, ligation was performed by addition of 2 μl of T4 RNA Ligase 2 (truncated K227Q), 2 μl T4 RNA Ligase 2 buffer and 6 μl PEG8000 (all components from NEB) and incubation at 14 °C ON. ..

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